The redundancy plot is another way to analyze digestion data. It is specifically useful for digestion by non-specific enzymes where overlapping peptides are produced. In some sense, it takes the Cleavage map and compresses it into one line. It is thus suitable for comparing complex data, including numerous experimental conditions, large proteins, and digests with high redundancy.
The x-axis represents the protein sequence, and the y-axis represents the redundancy (how many times each residue is covered by a peptide). For an ideal tryptic digest producing peptides covering the entire sequence, there will be a straight line at the value 1. The line will go up and down for non-specific digest with many overlapping peptides. The optimal state is to have the fluctuation minimal. This plot provides additional insight into the redundancy distribution behind the average value shown in the Statistics window in the Sidebar on the left. This value is shown in the plot as a dashed line.