Laboratory of Structural Biology and Cell Signaling

2022/07/21:
Our work on top-down FPOP, highlighting the benefits of exact gas-phase isolation of selected protein species and multiple fragmentation modes, is now available in the Analytical Chemistry!
2022/06/22:
Another popularization event with Aleš & Co. participation - Věda Fest!
2022/06/10:
Congratulations to Aleš on becoming a group leader at the 1st Faculty of Medicine where he will continue with Víťa in their adventures. Good luck! We enjoyed the time together and are glad that we stay in close contact and will still work together.
2022/06/04:
Our PhDs lead by Aleš were presenting at the Science Fair (Veletrh vědy). Great event! Some pictures here - one, two, three... ;)
2022/06/02:
Another great paper on NQO1, now about cavity making mutantions and allostery in flavoproteins. Excellent multilaboratory collaboration lead by Angel L. Pey from the University of Granada. Thanks to EPIC-XS H2020 Project for support!
2022/05/19:
This is big. Fantastic collaboration with Moshe and Yoni on cis-prenyltransferase continues with another great outcome. Paper now published in Sci. Adv. reveals how these enzymes synthesize long products via small active site!

2022/07/22:
Synopsis of Ales' Hippostruct MSCA project is available here.
2022/04/22:
Are you interested in structural mass spectrometry, method development ad/or membrane proteins? We are looking for Bc, MSc, PhD students and post-doc! Inquiries should be addressed to Petr Novak.

Downloads

Software for interpretation of high-resolution MS data obtained after protein chemical cross-linking. The software is free to use. If you are using LinX for your research please cite the related publication.

Download here

This software is part of a project that has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 731077P.

HDX-MS related tools for data processing and visualization.
Digestion_metrics - Microsoft Excel macro calculating simple digestion metrics (coverage, average peptide length, redundancy, etc.) Modified supplementary file to publication Kadek A et al Anal Chem. 2014, 86(9):4287-94.
HDXPeptideSplitter - Python script for pre-processing of HDX-MS results before using them in PyMol. Processed data are then loaded through data2bfactor Pymol script written by Robert L. Campbell. This version works with Python version 2.x. For version 3.x and higher use this modified data2bfactor script.