Laboratory of Structural Biology and Cell Signaling

2023/09/14:
Great news! Our JACS paper on FFAP technology was awarded Hanus Prize 2023 Congratulations to Kuky, Luky and all others.
2023/09/07:
New MMB chapter on our HDX workflow full of tips and tricks, especially on immobilized protease columns. This time applied to antibodies and from Zuza.
2023/08/25:
Another masterpiece from the ongoing collaboration with the Veverka group!
2023/07/18:
Good bye Maartje, thank you for teaching us 2DMS and good luck in Rouen!
2023/06/29:
Zuza's lucky week - state doctoral exam done and second paper out!
2023/06/01:
Congratulations to Valča on a successful MSc defense and good luck in future PhD!
2023/05/15:
Congratulations to Terka on the best IMIC master thesis in 2022!
2023/05/15:
MALDI chips rocks! Pepa's first paper is out in Clinical Proteomics!
2023/04/13:
Thrilling collaboration with Klara Hlouchova on “non-existing proteins”. Our achievements got well accepted in Nat Ecol Evol. Thanks EPIC-XS for support.
2023/04/12:
And yet another one piece with Angel Pey and Milagros Medina. Our collaborative effort on NQO1 active site mutations is now fully published in FEBS J. And again thanks EU FT-ICR MS (H2020) for support.

2023/02/20:
We are looking for java programmer. Position for two years. Collaboration with industrial partner - Bruker Daltonics! More details HERE.
2022/07/22:
Synopsis of Ales' Hippostruct MSCA project is available here.
2022/04/22:
Are you interested in structural mass spectrometry, method development ad/or membrane proteins? We are looking for Bc, MSc, PhD students and post-doc! Inquiries should be addressed to Petr Novak.

Downloads

Software for interpretation of high-resolution MS data obtained after protein chemical cross-linking. The software is free to use. If you are using LinX for your research please cite the related publication.

Download here

This software is part of a project that has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 731077P.

HDX-MS related tools for data processing and visualization.
Digestion_metrics - Microsoft Excel macro calculating simple digestion metrics (coverage, average peptide length, redundancy, etc.) Modified supplementary file to publication Kadek A et al Anal Chem. 2014, 86(9):4287-94.
HDXPeptideSplitter - Python script for pre-processing of HDX-MS results before using them in PyMol. Processed data are then loaded through data2bfactor Pymol script written by Robert L. Campbell. This version works with Python version 2.x. For version 3.x and higher use this modified data2bfactor script.