News:

  • 2025/07/07:

    Great and fruitful collaboration with the Haitin group, this time on GGPPS (aka "another prenyl transferase"). Inspiring work on enzyme oligomerization using HDX and native MS. And a second PhD paper for Jasmin! 🎉

  • 2025/06/25:

    Congrats to Pepa 🎉 👏 on a successful PhD defense. Good luck in your furture steps Pepa and thanks for all the fun!

  • 2025/06/25:

    We are organizing a hands-on Structural MS workshop supported by the OP JAK – Photomachines project and the SPIDoc's doctoral network. This is a unique opportunity to learn sample preparation, analysis, and data processing in HDX-MS, native MS, top-down MS, and cross-linking MS - topped off with a touch of ultra-high-resolution mass spectrometry! Don’t miss your chance to register. The workshop will take place on September 15–19, 2025, at BIOCEV.

  • 2025/06/05:

    Great news! 🎉 👏 Ljubi has successfully defended her PhD thesis. Well done, Doctor!

  • 2025/05/29:

    Congratulations to Kuba on successfully defending his master’s thesis! The gate to a PhD is now open!

  • 2025/05/11:

    Well, it took some time, but we’ve finally brought HDX analysis of the Cl⁻/H⁺ antiporter to a high-resolution, high-throughput level, allowing analysis of numerous conditions and pH-matching time points — and the timing couldn’t have been better. Our findings align beautifully with cryo-EM structures from groups at Stanford and offer key insights into the mechanism of chloride/proton transport. The preprint is now live on BioRxiv. Great work, Jasmin and Luky!

  • 2025/05/11:

    That was the first time we started doing fast HDX at low temperature and were locked with Paja in a cold room - cis-PT times. Now the story goes out through a collaborative effort of three groups, led by the Haitin group! Nature *Whatever*, here we come!

  • 2025/04/30:

    Alan's work on coupling a q-TOF mass spectrometer to a synchrotron is now out in Phys. Chem. Chem. Phys.. Check out how protein ions can be dissociated using tools not typically accessible in mass spectrometry.

  • 2025/04/25:

    Get ready to take the next step in your career journey! Join us for the SPIDoc's Career Day, connect with professionals and discover diverse career pathways. When: May 16, 2025. Where: Biocev, CZ or online. See more details in the flyer.

  • 2025/04/16:

    A new paper, led by our current and former students (Valča, Rosi and Michael) shows how combining novel isotopically labeled, MS-cleavable cross-linkers with DIA-MS acquisition improves the description of structural changes in therapeutic proteins.

EC
OPJAK
GACR TACR
BlueSky     Twitter!     YouTube

Important:

People

Laboratory is located in the BIOCEV center in Vestec. You can find us in labs J1.011-13, D2.004 and offices J1.006-008, D2.011.

Alumni

  • David ADÁMEK (MSc 2012) – Medical Manager at Roche and Podcaster!
  • Vítězslav BRINSA (PhD 2020-22) working with Aleš at the 1st Faculty of Medicine, Charles University
  • Martin ČONKA (MSc 2012) – Research Fellow at ExBio
  • Lada DLABOLOVÁ (MSc 2024)
  • Josef DVOŘÁK (PhD 2025)
  • Jan FIALA (PhD 2021) – Postdoctoral Fellow at Albert Heck's lab (Utrecht University, NL)
  • František FILANDR (PhD 2020) – Postdoctoral Fellow at the University of Calgary
  • Růžena FILANDROVÁ (LIŠKOVÁ) (PhD 2021) – Postdoctoral Fellow at the University of Calgary + 👶
  • Barbora FILIPOVÁ (Bc 2018) – chasing 🦘
  • Lukáš FOJTÍK (PhD 2024) – R&D at SOTIO
  • Pavel HANČ (MSc 2011) – Postdoctoral Fellow at Harvard Medical School
  • Jiří HAUSNER (MSc 2014)
  • Aleš HNÍZDA (junior scientist 2020-22) - Group leader at the 1st Faculty of Medicine, Charles University
  • Barbora JIREČKOVÁ (MSc 2022) - faculty hospital Vinohrady
  • Tereza KADAVÁ (MSc 2022) – pursuing PhD at at Albert Heck's lab (Utrecht University, NL)
  • Alan KÁDEK (PhD 2016) – Postdoctoral Fellow at Heinrich Pette Institute & European XFEL, Hamburg (🍔+🍺+⚓) till 8/2021. Now back home! (btw, should this be in the Alumni section? 😵)
  • Kristýna KOTÝNKOVÁ (MSc 2011) – Postdoctoral Researcher at Boston University School of Medicine
  • Veronika KÚDELOVÁ (Bc 2019)
  • Jan KUKLA (MSc 2014) – PRO.MED.CS
  • Zuzana MATOUŠKOVÁ (MSc 2020)
  • Alexander MELIKOV (MSc 2024)
  • Hynek MRÁZEK (post-doc 2012-2014) – IQVIA RDS
  • Kamila PELECHOVÁ (Bc 2019)
  • Marek POLÁK (PhD 2024) – playing with gators and huge magnets at the National High Magnetic Field Laboratory, Florida
  • Eliška POSPÍŠILOVÁ (MSc 2012)
  • Valérie PROCHÁZKOVÁ (Bc/MSc 2023) – 🍷 🐹 🍸
  • Michal ROSŮLEK (PhD) – researcher at Lonza
  • Daniel ROZBESKÝ (PhD 2013) – Junior group leaded at Charles University (BioCeV) after nice Post-doc at Oxford
  • Jana ŘEHÁKOVÁ (Bc 2019) - brewing 🍺
  • Kristián SKÁLA (MSc 2017)
  • Lukáš SLAVATA (PhD) – representative at HSPT s.r.o. / Agilent
  • Jitka SMETANOVÁ (MSc 2018)
  • Petra SVOBODOVÁ (DAREBNÁ) (MSc 2015)
  • Šárka SVOBODOVÁ (Bc 2018)
  • Kristýna ŠINTÁKOVÁ (Bc 2019)
  • Vyacheslav TRETYACHENKO (Bc 2013)
  • Karel VALIŠ (post-doc 2011-2021)
  • Maria A. VAN AGTHOVEN (senior scientist 2021-2023) – scientist at the Université de Rouen
  • Pavla VAŇKOVÁ (PhD 2020) – Staff scientist at IBT CAS / BioCeV - Center for Molecular Structure
  • František VOSTÁREK (MSc 2012)
  • Lucie WALLENFELS (HERNYCHOVÁ) (PhD 2019) – PharmInvent/PrimeVigilance
  • Ghazaleh YASSAGHI (post-doc 2017-2019) – Pfizer
  • Romana ZÁHUMENSKÁ (MSc 2017)
  • Eva ZÁKOUCKÁ (MSc 2014) – ⛷ ☕ 🥤 🧁 🍰
  • Valentyn II. THE BETTA FISH (lab fish). We keep memorries of your beauty.
  • Mike I. - THE ITSY BITSY SPIDER (lab spider) – currently catching 🪰 in 🕷 ☁️ and singing his favourite song