News:

  • 2025/05/11:

    Well, it took some time, but we’ve finally brought HDX analysis of the Cl⁻/H⁺ antiporter to a high-resolution, high-throughput level, allowing analysis of numerous conditions and pH-matching time points — and the timing couldn’t have been better. Our findings align beautifully with cryo-EM structures from groups at Stanford and offer key insights into the mechanism of chloride/proton transport. The preprint is now live on BioRxiv. Great work, Jasmin and Luky!

  • 2025/05/11:

    That was the first time we started doing fast HDX at low temperature and were locked with Paja in a cold room - cis-PT times. Now the story goes out through a collaborative effort of three groups, led by the Haitin group! Nature *Whatever*, here we come!

  • 2025/04/30:

    Alan's work on coupling a q-TOF mass spectrometer to a synchrotron is now out in Phys. Chem. Chem. Phys.. Check out how protein ions can be dissociated using tools not typically accessible in mass spectrometry.

  • 2025/04/25:

    Get ready to take the next step in your career journey! Join us for the SPIDoc's Career Day, connect with professionals and discover diverse career pathways. When: May 16, 2025. Where: Biocev, CZ or online. See more details in the flyer.

  • 2025/04/16:

    A new paper, led by our current and former students (Valča, Rosi and Michael) shows how combining novel isotopically labeled, MS-cleavable cross-linkers with DIA-MS acquisition improves the description of structural changes in therapeutic proteins.

  • 2025/04/03:

    Great news! Our MSCA Doctoral Network proposal, ProtAIomics, has successfully passed the evaluation stage and is now invited for Grant Agreement Preparation. We look forward to contributing to this pan-European collaboration aimed at making mass spectrometry data interpretation less tedious.

  • 2025/04/01:

    Growing again. New PhD student Marta Cruz 🇵🇹; has already been there for a short stay. Now she returned for several years. Good luck with your PhD project and welcome Marta!

  • 2025/03/31:

    Some fantastic news! Friday, March 28, was a great day for our three PhDs – Marek🎉 👏, Michal🎉 👏, and Lukáš🎉 👏 – as they successfully defended their theses. Congratulations to our new doctors, and thank you for all your work and outstanding presentations!

  • 2025/03/31:

    Let’s talk about… well, 💩 . It might not sound pleasant, but our MALDI chip test significantly speeds up the detection of bacteria responsible for severe gut infections—preventing complications that could lead to systemic sepsis. Faster results lead to better treatment and improved patient outcomes.

  • 2025/03/24:

    Great news for barbecue lovers. Say goodbye to worries about botulism poisoning—thanks to our MS-based test that makes Botulinum detection faster and more affordable. This simple method is 100 times quicker, cheaper than the standard approach and, best of all, requires no animal testing!

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Important:

About laboratory


The Laboratory of Structural Biology and Cell Signaling is a multidisciplinary team focused on two main research directions.
The structural biology group specializes in recombinant protein expression and the structural and dynamic characterization of proteins and protein–ligand complexes. They employ advanced mass spectrometric techniques, including native mass spectrometry, hydrogen–deuterium exchange, chemical and radical footprinting, and protein cross-linking. Beyond applying these methods, the group also actively develops new enzymatic columns for online protein digestion and novel labeling reagents for surface modification and cross-linking. Due to the complexity of the resulting data and the scarcity of commercial tools for analysis, the group invests substantial effort into building customized software solutions.
The clinical proteomics unit explores the molecular basis of a broad spectrum of human diseases—from bacterial and viral infections to diabetes, kidney dysfunction, and neurodegenerative conditions such as dementia. One of the unit’s core strengths lies in its proprietary technology for fabricating protein chips designed for immunoaffinity enrichment of specific proteins from biological fluids, including plasma, serum, and cerebrospinal fluid. These chips are fully compatible with mass spectrometric analysis, enabling highly sensitive, specific, and robust protein quantification. This technology is well aligned with the vision of personalized medicine, offering early diagnostic capabilities that can reduce treatment costs and improve patient outcomes and productivity.
The close collaboration between these two groups creates a unique research platform where biological insights can be validated and understood at the molecular level. The laboratory is located at the BIOCEV center in Vestec.

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